Research Associate I, CFPS Production

BigHat Biosciences is seeking a self-motivated individual for a Research Associate, CFPS Production role to manufacture, validate and execute experiments to develop cell-free protein synthesis (CFPS) reagents for BigHat's high-throughput, automated platform for producing and characterizing antibodies.

Responsibilities

Manufacture and validate CFPS reagents used in BigHat's high-throughput antibody screening platform including making buffers, media for biomass production and performing QC experiments on reagents; Maintain CFPS reagent manufacturing equipment including incubators, centrifuges, and homogenizers; Coordinate with lab ops team to request/order supplies for CFPS reagent manufacturing to ensure reliable stock for platform; Design and run experiments for development of next generation CFPS extract to optimize yield and purity of next generation antibodies on our platform; Keep a detailed record of all experiments using BigHat's documentation tools, and clearly communicate results and challenges; Present experimental plans and results during regular meetings with manager and science teams

Requirements

B.S. degree in Biochemistry, Molecular Biology, or related field with 1-2 years of industry experience; Experience working with recombinant protein expression from bacterial cultures; Experience with molecular cloning techniques including primer design, PCR, mutagenesis, Golden Gate Assembly; Excellent communication skills; organized and works well in small groups

Nice to have

Experience with CFPS; Experience and knowledge of protein purification methods including affinity chromatography and SEC; Experience with quantitative and/or statistical analysis software (e.g. Excel, GraphPad Prism, Python, R); Experience with automation and high-throughput tools and robotic instrumentation

Benefits

Bonus; Options; Range of health insurance plan options through Anthem and Kaiser (monthly credit if benefit waived); Dental and vision coverage through Guardian; Additional well-being benefits through Nayya, OneMedical, Wagmo, Rula and more; 401(k) with company match; DTO, two weeks of company-wide shutdown, and 12 company holidays; Paid parental leave