Scientist / Senior Scientist, Library Prep

Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform. The Scientist or Senior Scientist will lead research on the library preparation stage of the platform, owning how DNA molecules produced by the protein sequencing assay (ProSE) are prepared for and read on the nanopore. This is a full-time, exempt, in-person position at the Berkeley, CA location. Reporting to the Director of Assay Development.

Responsibilities

Own library prep method development for nanopore sequencing end to end (multiplexing, sample barcoding, second-strand synthesis, end repair, adapter ligation); Own the steps at the interface between the protein sequencing assay and library prep; Develop on-bead preparation and clean-up methods for high-sensitivity sequencing from low-input material, with characterization assays; Feed library prep requirements back into the design of upstream constructs and oligos; Contribute to experimental design for ProSE studies and sequencing runs, and move proven manual workflows onto automated platforms; Investigate and resolve yield-limiting artifacts and DNA library characteristics that reduce yield or bias results, using computational tools (SnapGene, mFold, NUPACK) and bench validation; Use sequencing data to correlate library prep variables with read counts, pore health, and classification accuracy; Provide technical guidance and experiment design to team members running routine library prep; Work with oligo synthesis, assay development, integration, automation, and bioinformatics teams; Maintain rigorous documentation

Requirements

PhD in Molecular Biology, Biochemistry, Bioengineering, Chemical Biology, or a related field, with 1+ years of industry experience, or an MS with 5+ years of relevant experience; Demonstrated experience developing or substantially re-engineering a sequencing library prep method for demanding templates (e.g., low-input, PCR-free, single-molecule, single-stranded, or damaged DNA); Deep understanding of enzymatic DNA manipulation (ligation, end repair, phosphorylation, polymerase extension, adapter attachment); Experience working with chemically modified DNA or RNA and troubleshooting nucleic acid secondary structure; End-to-end systems thinking across upstream chemistry, construct design, and sequencing hardware; Ability to reason from first principles to predict reagent and condition efficacy; Proficiency with DNA characterization and QC methods such as capillary or gel electrophoresis; Experience guiding the bench work of others; Excellent problem-solving skills and attention to detail; Excellent written and verbal communication skills; Ability to thrive in a fast-paced environment with evolving priorities

Nice to have

Hands-on experience with nanopore sequencing or another single-molecule DNA sequencing technology such as PacBio; Experience characterizing the electrochemical properties of nanopores or related systems (e.g., ion channels, nanoelectrodes); Experience with single-stranded DNA or RNA library prep, or PCR-free preparation with modified oligonucleotides in enzymatic workflows; Familiarity with automated liquid handling platforms (such as Hamilton, KingFisher); Background in protein sequencing, proteomics sample preparation, or peptide chemistry, including oligo-peptide conjugate characterization (LCMS, HPLC); Comfort using AI tools (Claude, Claude Code) to accelerate literature review, protocol design, and data analysis

Benefits

Employee Stock Option Plan; 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision); Employer Retirement Contributions to 401(k); Generous Paid Time Off; Paid Maternity and Paternity Leave; Health & Wellbeing Program; Office Snacks and Beverages; Regular Team Bonding Activities