Automation Cell Culture Biologist

Axle is seeking a Automation Cell Culture Biologist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute on Aging (NIA) located in Bethesda, MD. This automated cell culture biologist supports research at the Center for Alzheimer's and Related Dementias by characterizing genetic pathways for disease by automated large scale cell culture experiments. Provides technical experience needed to assist in studying the basic principles of plant and animal life.

Responsibilities

Supports research at the Center for Alzheimer's and Related Dementias by characterizing genetic pathways for disease by automated large scale cell culture experiments; Plan and administer biological research programs; Develop and maintain liaisons and effective working relations with groups and individuals, agencies, and the public; Characterize genetic pathways for Alzheimer's disease and related dementia (ADRD) using human cell-based models; Perform large-scale iPSC cell culture experiments on liquid handling platforms; Program, operate, and implement novel methods on liquid handling platforms (Hamilton, Tecan); Perform experiments and procedures related to differentiation and phenotyping of induced pluripotent stem cell lines; Evaluate the stability of iPSC and iPSC-derived cells across multiple engineered and non-engineered lines; Apply published protocols for cell differentiation to iPSC lines, microscopy, and molecular analysis; Work independently in designing, developing, and performing new methods; Provide insight into the effects of ADRD-associated mutations on molecular readouts in cell culture; Support other research projects; Collect, analyze, interpret, and organize data and present results in team and departmental meetings; Keep detailed, organized, and accurate digital and physical experimental records; Update and maintain laboratory notebooks and databases; Coordinate the work of biological technicians and technologists and other scientists; Collaborate with other team members to carry out large-scale cell culture protocols using high throughput robotic systems; Perform standard immunological techniques, including western blotting and immunostaining; Operate, calibrate, or maintain equipment used in quantitative or qualitative analysis; Maintain laboratory equipment; Train staff on the proper usage of laboratory instruments and equipment; Calibrate, maintain, and troubleshoot instruments and equipment in the laboratory; Develop new automated differentiation protocols for iPSC lines to differentiate into disease-relevant cell types like neurons, astrocytes, and microglia; Develop image analysis routines to analyze the effects of disease-associated mutations on cellular phenotypes

Requirements

Good Laboratory Practices (GLP); Master's degree in cell biology, molecular biology, genetics, biochemistry, bioengineering, chemical engineering, or a closely related discipline; Minimum of two (2) years of related experience; Genetics; Neuroscience; Molecular Biology; Outlook; R; Seurat; Excel; Power point; Google Drive; Experience in programming/coding (VENUS, Visual Basic, C++, MATLAB, or Python programming); Proficiency with automated liquid handling robots; Planning and optimizing experiments; QC Testing; Hands-on experience with cell culture in particular working with iPS cells; Hands-on experience with operating and programming liquid handling robotics and platforms (Hamilton preferred, TECAN, Beckman, Agilent); Knowledge of proper laboratory disposal methods and procedures; Strong communications skills, both oral and written; Excellent analytical, organizational and time management skills

Nice to have

Hamilton preferred

Benefits

100% Medical, Dental & Vision Coverage for Employees; Paid Time Off and Paid Holidays; 401K match up to 5%; Educational Benefits for Career Growth; Employee Referral Bonus; Flexible Spending Accounts: Healthcare (FSA); Parking Reimbursement Account (PRK); Dependent Care Assistant Program (DCAP); Transportation Reimbursement Account (TRN)