Principal/Senior Scientist, mRNA engineering

NewLimit is seeking a scientist to drive mRNA production and engineering efforts. This is a hands-on, lab based role focused on the synthesis, purification, and characterization of mRNA at large scales and high purities. You will serve as the technical lead for our production workflow, bridging the gap between molecular design and high quality material generation for in vivo and in vitro studies.

Responsibilities

Execute and scale end-to-end mRNA production, including template preparation, in vitro transcription (IVT), and purification (TFF, chromatography); Implement rigorous quality control assays, including direct RNA sequencing, fragment analysis, dsRNA quantitation, and residual contaminant analysis; Author and maintain detailed SOPs and batch records, ensuring all production runs meet GLP standards; Independently design and execute experiments to improve mRNA sequence potency, specificity, and manufacturing performance; Train, manage, and mentor other team members on mRNA optimization and production workflows

Requirements

PhD in molecular biology, biochemistry, cell biology, or a related field or equivalent industry experience (5+ years); Deep hands-on experience with IVT and the specific challenges of scaling nucleic acid production; Proficiency with TFF and chromatography systems for the purification of large scale RNA batches; Ability to perform and interpret mRNA QC assays and troubleshoot mRNA production challenges; Strong experience in drafting technical reports and standardized protocols

Nice to have

Familiarity with Python or R for sequence analysis and secondary structure prediction; Understanding of how mRNA quality attributes impact Lipid Nanoparticle (LNP) formulation and delivery; Experience with complex plasmid design and bacterial scale-up for DNA template generation; Experience managing external contract manufacturing organizations (CMOs)

Benefits

Health, dental, and vision insurance; 401(k) with company match; Paid parental leave; Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays; Meals and snacks on-site